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The two protein bands from 40 kDa to thirty kDa were absent immediately after 24 h incubation at 45 °C. For the remainder of the bands, numerous concentrations of all 3 proteases retained protein bands corresponding to their authentic molar masses (Determine 6a,b). The existence of bands at small molecular weight in fish roe prior to hydrolysis was unforeseen. From the report by Ghelichi et al. [8], minor peaks of a relatively reduced molecular bodyweight selection (3–fourteen kDa) were being observed in defatted carp roe homogenate, probably on account of an autolytic course of action occurring in fish roe and/or the defatting procedure leading to the formation of low-molecular-excess weight peptides.
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